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SLITRK6 Polyclonal antibody

SLITRK6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21150-1-AP

Synonyms

SLIT and NTRK like protein 6, SLITRK6

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using SLITRK6 Polyclonal antibody (21150-1-AP)

    WB analysis of A549 using 21150-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21150-1-AP (SLITRK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using SLITRK6 Polyclonal antibody (21150-1-AP)

    WB analysis of mouse lung using 21150-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21150-1-AP (SLITRK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLITRK6 Polyclonal antibody (21150-1-AP)

    IF Staining of HepG2 using 21150-1-AP

    Immunofluorescent analysis of HepG2 cells, using SLITRK6 antibody 21150-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inA549 cells, mouse lung tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21150-1-AP is SLITRK6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLITRK6 fusion protein Ag15701
Full Name SLIT and NTRK-like family, member 6
Calculated Molecular Weight 841 aa, 95 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC101070
Gene Symbol SLITRK6
Gene ID (NCBI) 84189
RRIDAB_10732812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLITRK6 is a member of the SLITRK family. Members of this family are integral membrane proteins that are characterized by two N-terminal leucine-rich repeat (LRR) domains and a C-terminal region that shares homology with trk neurotrophin receptors. This protein functions as a regulator of neurite outgrowth required for normal hearing and vision. Mutations in SLITRK6 gene are a cause of myopia and deafness.

Protocols

Product Specific Protocols
WB protocol for SLITRK6 antibody 21150-1-APDownload protocol
IF protocol for SLITRK6 antibody 21150-1-APDownload protocol
FC protocol for SLITRK6 antibody 21150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols