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SSTR1 Polyclonal antibody

SSTR1 Polyclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

Cat no : 20587-1-AP

Synonyms

SS1 R, SS 1 R, SRIF-2, SRIF 2, Somatostatin receptor type 1

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using SSTR1 Polyclonal antibody (20587-1-AP)

    WB analysis of L02 using 20587-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using SSTR1 Polyclonal antibody (20587-1-AP)

    WB analysis of mouse small intestine using 20587-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SSTR1 Polyclonal antibody (20587-1-AP)

    WB analysis of mouse brain using 20587-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using SSTR1 Polyclonal antibody (20587-1-AP)

    WB analysis of BxPC-3 using 20587-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using SSTR1 Polyclonal antibody (20587-1-AP)

    WB analysis of SH-SY5Y using 20587-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using SSTR1 Polyclonal antibody (20587-1-AP)

    FC experiment of SH-SY5Y using 20587-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug SSTR1 Polyclonal antibody (20587-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inL02 cells, BxPC-3 cells, mouse brain tissue, mouse small intestine tissue, SH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20587-1-AP targets SSTR1 in WB, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSTR1 fusion protein Ag14287
Full Name somatostatin receptor 1
Calculated Molecular Weight 391 aa, 43 kDa
Observed Molecular Weight 53 kDa, 63 kDa
GenBank Accession NumberBC035618
Gene Symbol SSTR1
Gene ID (NCBI) 6751
RRIDAB_10755146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SSTR1 antibody 20587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols