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WDR6 Polyclonal antibody

WDR6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20508-1-AP

Synonyms

WD repeat-containing protein 6, WD repeat domain 6, WD repeat containing protein 6, tRNA (34-2'-O)-methyltransferase regulator WDR6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using WDR6 Polyclonal antibody (20508-1-AP)

    WB analysis of HeLa using 20508-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20508-1-AP (WDR6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using WDR6 Polyclonal antibody (20508-1-AP)

    WB analysis of HeLa using 20508-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20508-1-AP (WDR6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using WDR6 Polyclonal antibody (20508-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 20508-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 20508-1-AP (WDR6 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WDR6 Polyclonal antibody (20508-1-AP)

    IF Staining of HepG2 using 20508-1-AP

    Immunofluorescent analysis of HepG2 cells, using WDR6 antibody 20508-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:25-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20508-1-AP targets WDR6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR6 fusion protein Ag14316
Full Name WD repeat domain 6
Calculated Molecular Weight 1121 aa, 122 kDa
Observed Molecular Weight 122 kDa
GenBank Accession NumberBC002826
Gene Symbol WDR6
Gene ID (NCBI) 11180
RRIDAB_10732597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WDR6 antibody 20508-1-APDownload protocol
IHC protocol for WDR6 antibody 20508-1-APDownload protocol
IF protocol for WDR6 antibody 20508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols