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TADA1L Polyclonal antibody

TADA1L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20337-1-AP

Synonyms

TADA1, STAF42, SPT3-associated factor 42, SPT3 associated factor 42, RP1 9E21.4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using TADA1L Polyclonal antibody (20337-1-AP)

    WB analysis of mouse testis using 20337-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20337-1-AP (TADA1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using TADA1L Polyclonal antibody (20337-1-AP)

    WB analysis of NIH/3T3 using 20337-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20337-1-AP (TADA1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TADA1L Polyclonal antibody (20337-1-AP)

    IHC staining of human testis using 20337-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20337-1-AP (TADA1L Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TADA1L Polyclonal antibody (20337-1-AP)

    IHC staining of human testis using 20337-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20337-1-AP (TADA1L Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TADA1L Polyclonal antibody (20337-1-AP)

    IF Staining of HepG2 using 20337-1-AP

    Immunofluorescent analysis of HepG2 cells using 20337-1-AP (TADA1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, NIH/3T3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20337-1-AP targets TADA1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TADA1L fusion protein Ag14118
Full Name transcriptional adaptor 1 (HFI1 homolog, yeast)-like
Calculated Molecular Weight 335 aa, 37 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC015401
Gene Symbol TADA1L
Gene ID (NCBI) 117143
RRIDAB_2878675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.The MW of this protein is 37 kDa, and Catalog#20337-1-AP specially recognises the 37 kDa protein.

Protocols

Product Specific Protocols
WB protocol for TADA1L antibody 20337-1-APDownload protocol
IHC protocol for TADA1L antibody 20337-1-APDownload protocol
IF protocol for TADA1L antibody 20337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genes Dev

The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma

Authors - Ying-Jiun C Chen
View Article