• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MEF2C Polyclonal antibody

MEF2C Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 20326-1-AP

Synonyms

MEF2C, myocyte enhancer factor 2C

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of HeLa using 20326-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of SH-SY5Y using 20326-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of mouse testis using 20326-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of mouse small intestine using 20326-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lymph tissue using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of rat lymph using 20326-1-AP

    rat lymph tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using MEF2C Polyclonal antibody (20326-1-AP)

    IHC staining of human heart using 20326-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 20326-1-AP (MEF2C Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using MEF2C Polyclonal antibody (20326-1-AP)

    IHC staining of human heart using 20326-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 20326-1-AP (MEF2C Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MEF2C Polyclonal antibody (20326-1-AP)

    IF Staining of mouse brain using 20326-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (20326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MEF2C Polyclonal antibody (20326-1-AP)

    IF Staining of mouse brain using 20326-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (20326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse small intestine tissue, mouse testis tissue, rat lymph tissue, SH-SY5Y cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20326-1-AP targets MEF2C in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEF2C fusion protein Ag13255
Full Name myocyte enhancer factor 2C
Calculated Molecular Weight 469 aa, 51 kDa
Observed Molecular Weight45-70 kDa
GenBank Accession NumberBC026341
Gene Symbol MEF2C
Gene ID (NCBI) 4208
RRIDAB_10693772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.

Protocols

Product Specific Protocols
WB protocol for MEF2C antibody 20326-1-APDownload protocol
IHC protocol for MEF2C antibody 20326-1-APDownload protocol
IF protocol for MEF2C antibody 20326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols