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  • Featured Product
  • KD/KO Validated

BAG1 Polyclonal antibody

BAG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19064-1-AP

Synonyms

BAG1S/1M/1L, HAP, Bcl-2-associated athanogene 1, BAG1L speicfic, BAG-1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HeLa using 19064-1-AP

    WB result of BAG1 antibody (19064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG1 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HeLa using 19064-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of UV treated Hela using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of UV treated Hela using 19064-1-AP

    UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HT-1080 using 19064-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HL-60 using 19064-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of MCF-7 using 19064-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of Jurkat using 19064-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of MCF-7 using 19064-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HEK-293 using 19064-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of RAW264.7 using 19064-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    IP experiment of HeLa using 19064-1-AP

    IP result of anti-BAG1 (IP:19064-1-AP, 3ug; Detection:19064-1-AP 1:500) with HeLa cells lysate 3440ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human testis using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human testis using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human ovary using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human ovary using 19064-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    IF Staining of HeLa using 19064-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAG1 antibody (19064-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BAG1 Polyclonal antibody (19064-1-AP)

    IF Staining of MCF-7 using 19064-1-AP

    Immunofluorescent analysis of MCF-7 cells, using BAG1 antibody 19064-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, UV treated HeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells, RAW264.7, HT-1080 cells, HL-60 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19064-1-AP targets BAG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAG1 fusion protein Ag13555
Full Name BCL2-associated athanogene
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 50-55 kDa, 30-33 kDa
GenBank Accession NumberBC014774
Gene Symbol BAG1
Gene ID (NCBI) 573
RRIDAB_10640574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.

Protocols

Product Specific Protocols
WB protocol for BAG1 antibody 19064-1-APDownload protocol
IHC protocol for BAG1 antibody 19064-1-APDownload protocol
IF protocol for BAG1 antibody 19064-1-APDownload protocol
IP protocol for BAG1 antibody 19064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncol Rep

Magnetic gold nanoparticle-mediated small interference RNA silencing Bag-1 gene for colon cancer therapy.

Authors - Wenbai Huang
View Article
humanIHC

Ann Rheum Dis

Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration

Authors - Yue Fan
View Article
humanWB

Int Immunopharmacol

Construction of an autophagy-related genes risk model as predicting prognosis: BAG1 suppresses growth of clear cell renal cell carcinoma

Authors - Jiayu Guo
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Tongbin (Verified Customer) (08-25-2020)

This BAG1 antibody works very well in detecting endogenous BAG1 protein by western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart