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PIF1 Polyclonal antibody

PIF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19006-1-AP

Synonyms

C15orf20, PIF, PIF1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse thymus tissue using PIF1 Polyclonal antibody (19006-1-AP)

    WB analysis of mouse thymus using 19006-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19006-1-AP (PIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using PIF1 Polyclonal antibody (19006-1-AP)

    WB analysis of mouse thymus using 19006-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19006-1-AP (PIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19006-1-AP targets PIF1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIF1 fusion protein Ag13522
Full Name PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae)
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 63-73 kDa
GenBank Accession NumberBC018978
Gene Symbol PIF1
Gene ID (NCBI) 80119
RRIDAB_10598312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIF1, also named as ATP-dependent DNA helicase PIF1, is a 641 amino acid protein, which belongs to the helicase family. PIF1 as a DNA-dependent ATPase and 5'-3' DNA helicase is required for the maintenance of both mitochondrial and nuclear genome stability. PIF1 localizes in the nucleus and is involved in the maintenance of telomeric DNA. PIF1 exists as four isoforms. isoform 1 is a 70 kDa protein and isoform 2 is 63 kDa protein.

Protocols

Product Specific Protocols
WB protocol for PIF1 antibody 19006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

CircNEIL3 mediates pyroptosis to influence lung adenocarcinoma radiotherapy by upregulating PIF1 through miR-1184 inhibition.

Authors - Ting Zhang
View Article