• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PPIF-Specific Polyclonal antibody

PPIF-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18466-1-AP

Synonyms

Cyclophilin F, Cyp D, CYP3, peptidylprolyl isomerase F, PPIase F, PPIF, Rotamase F

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PPIF-Specific Polyclonal antibody (18466-1-AP)

    WB analysis using 18466-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using PPIF-Specific Polyclonal antibody (18466-1-AP)

    WB analysis of rat heart using 18466-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PPIF-Specific Polyclonal antibody (18466-1-AP)

    WB analysis of HepG2 using 18466-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using PPIF-Specific Polyclonal antibody (18466-1-AP)

    WB analysis of human heart using 18466-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using PPIF-Specific Polyclonal antibody (18466-1-AP)

    IP experiment of mouse heart using 18466-1-AP

    IP result of anti-PPIF-Specific (IP:18466-1-AP, 3ug; Detection:18466-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using PPIF-Specific Polyclonal antibody (18466-1-AP)

    IHC staining of human heart using 18466-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PPIF-Specific Polyclonal antibody (18466-1-AP)

    IHC staining of human heart using 18466-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PPIF-Specific Polyclonal antibody (18466-1-AP)

    IHC staining of human heart using 18466-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PPIF-Specific Polyclonal antibody (18466-1-AP)

    IHC staining of human heart using 18466-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PPIF-Specific Polyclonal antibody (18466-1-AP)

    IF Staining of MCF-7 using 18466-1-AP

    Immunofluorescent analysis of MCF-7 cells, using PPIF antibody 18466-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human heart tissue, rat heart tissue, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18466-1-AP targets PPIF-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIF-Specific fusion protein Ag13151
Full Name peptidylprolyl isomerase F
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005020
Gene Symbol PPIF
Gene ID (NCBI) 10105
RRIDAB_2169273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPIF, also named as CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF

Protocols

Product Specific Protocols
WB protocol for PPIF-Specific antibody 18466-1-APDownload protocol
IHC protocol for PPIF-Specific antibody 18466-1-APDownload protocol
IF protocol for PPIF-Specific antibody 18466-1-APDownload protocol
IP protocol for PPIF-Specific antibody 18466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury

Authors - Hakjoo Lee
View Article
humanIHC

Aging (Albany NY)

Overexpression of TICRR and PPIF confer poor prognosis in endometrial cancer identified by gene co-expression network analysis.

Authors - Linlin Yang
View Article
mouseWB

J Biol Chem

The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.

Authors - Hakjoo Lee
View Article
humanIF

PLoS One

Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM.

Authors - Wang Yun E YE
View Article
mouseWB

EMBO J

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses

Authors - Hao Liu
View Article
humanWB

Biol Pharm Bull

Cinnamtannin B-1 inhibits the progression of osteosarcoma by regulating the miR-1281/PPIF axis

Authors - Jun Jia
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Veronica (Verified Customer) (01-30-2024)

Really satisfied with the result in WB. No aspecific signal, clear band. Reccomended! Conditions: 12% acrylammide gel Ab’: 1:2000 in 5% milk O/N

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa
PPIF-Specific Antibody Western Blot validation (1:2000 dilution) in HeLa (Cat no:18466-1-AP)