• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MAPKBP1 Polyclonal antibody

MAPKBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17788-1-AP

Synonyms

JNK binding protein 1, JNKBP 1, JNKBP1, KIAA0596, MAPKBP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using MAPKBP1 Polyclonal antibody (17788-1-AP)

    WB analysis of mouse liver using 17788-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17788-1-AP (MAPKBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using MAPKBP1 Polyclonal antibody (17788-1-AP)

    WB analysis of rat liver using 17788-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 17788-1-AP (MAPKBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using MAPKBP1 Polyclonal antibody (17788-1-AP)

    IHC staining of human pancreas using 17788-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17788-1-AP (MAPKBP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using MAPKBP1 Polyclonal antibody (17788-1-AP)

    IHC staining of human pancreas using 17788-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17788-1-AP (MAPKBP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAPKBP1 Polyclonal antibody (17788-1-AP)

    IF Staining of HepG2 using 17788-1-AP

    Immunofluorescent analysis of HepG2 cells, using MAPKBP1 antibody 17788-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17788-1-AP targets MAPKBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPKBP1 fusion protein Ag11883
Full Name mitogen-activated protein kinase binding protein 1
Calculated Molecular Weight 1015 aa, 109 kDa, 164 kDa
Observed Molecular Weight 200-230 kDa
GenBank Accession NumberBC036660
Gene Symbol MAPKBP1
Gene ID (NCBI) 23005
RRIDAB_2235097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAPKBP1 antibody 17788-1-APDownload protocol
IHC protocol for MAPKBP1 antibody 17788-1-APDownload protocol
IF protocol for MAPKBP1 antibody 17788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols