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UTP15 Polyclonal antibody

UTP15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17416-1-AP

Synonyms

NET21, UTP15

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using UTP15 Polyclonal antibody (17416-1-AP)

    WB analysis of MCF-7 using 17416-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using UTP15 Polyclonal antibody (17416-1-AP)

    WB analysis of MCF-7 using 17416-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UTP15 Polyclonal antibody (17416-1-AP)

    WB analysis of HeLa using 17416-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UTP15 Polyclonal antibody (17416-1-AP)

    WB analysis of HeLa using 17416-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UTP15 Polyclonal antibody (17416-1-AP)

    WB analysis of HepG2 using 17416-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17416-1-AP targets UTP15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UTP15 fusion protein Ag11161
Full Name UTP15, U3 small nucleolar ribonucleoprotein, homolog (S. cerevisiae)
Calculated Molecular Weight285aa,32 kDa; 518aa,58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC013064
Gene Symbol UTP15
Gene ID (NCBI) 84135
RRIDAB_2304317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UTP15 is a U3 snoRNA-associated protein of the Small Subunit Processome (SSU), essential for 18S rRNA biogenesis. UTP15 has 3 isoforms with MW 56, 58 and 37kDa (refer to UniProt). Catalog#17416-1-AP is a rabbit polyclonal antibody raised against the full-length of human UTP15. It specially recognizes 58 and 37kDa isoforms.

Protocols

Product Specific Protocols
WB protocol for UTP15 antibody 17416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols