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PRPF40B Polyclonal antibody

PRPF40B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 16929-1-AP

Synonyms

Huntingtin yeast partner C, HYPC, PRPF40B

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using PRPF40B Polyclonal antibody (16929-1-AP)

    WB analysis of Y79 using 16929-1-AP

    WB result of PRPF40B antibody (16929-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRPF40B transfected Y79 cells.

  • Western Blot (WB) analysis of various lysates using PRPF40B Polyclonal antibody (16929-1-AP)

    WB analysis using 16929-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16929-1-AP (PRPF40B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using PRPF40B Polyclonal antibody (16929-1-AP)

    WB analysis of Y79 using 16929-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 16929-1-AP (PRPF40B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using PRPF40B Polyclonal antibody (16929-1-AP)

    WB analysis of Y79 using 16929-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 16929-1-AP (PRPF40B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using PRPF40B Polyclonal antibody (16929-1-AP)

    IP experiment of Y79 using 16929-1-AP

    IP result of anti-PRPF40B (IP:16929-1-AP, 4ug; Detection:16929-1-AP 1:5000) with Y79 cells lysate 2800 ug.

Tested Applications

Positive WB detected inY79 cells, K-562 cells, mouse testis tissue
Positive IP detected inY79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

16929-1-AP targets PRPF40B in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRPF40B fusion protein Ag10361
Full Name PRP40 pre-mRNA processing factor 40 homolog B (S. cerevisiae)
Calculated Molecular Weight 871 aa, 99 kDa
Observed Molecular Weight130-150 kDa
GenBank Accession NumberBC067364
Gene Symbol PRPF40B
Gene ID (NCBI) 25766
RRIDAB_2171041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRPF40B antibody 16929-1-APDownload protocol
IP protocol for PRPF40B antibody 16929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nucleic Acids Res

PRPF40A induces inclusion of exons in GC-rich regions important for human myeloid cell differentiation

Authors - Cheryl Weiqi Tan
View Article