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LMF1 Polyclonal antibody

LMF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16812-1-AP

Synonyms

C16orf26, HMFN1876, JFP11, lipase maturation factor 1, LMF1, TMEM112, TMEM112A, Transmembrane protein 112

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using LMF1 Polyclonal antibody (16812-1-AP)

    WB analysis of SKOV-3 using 16812-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16812-1-AP (LMF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LMF1 Polyclonal antibody (16812-1-AP)

    IHC staining of human liver cancer using 16812-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16812-1-AP (LMF1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LMF1 Polyclonal antibody (16812-1-AP)

    IHC staining of human liver cancer using 16812-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16812-1-AP (LMF1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inSKOV-3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 16812-1-AP is LMF1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMF1 fusion protein Ag10349
Full Name lipase maturation factor 1
Calculated Molecular Weight 567 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC010738
Gene Symbol LMF1
Gene ID (NCBI) 64788
RRIDAB_2878320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LMF1, also named as C16orf26 and TMEM112, is involved in the maturation of lipoprotein lipase (LPL) and hepatic lipase in endoplasmic reticulum. It is required for maturation and transport of active lipoprotein lipase (LPL) through the secretory pathway.

Protocols

Product Specific Protocols
WB protocol for LMF1 antibody 16812-1-APDownload protocol
IHC protocol for LMF1 antibody 16812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Legal Med

Candidate biomarkers in brown adipose tissue for post-mortem diagnosis of fatal hypothermia

Authors - Miao Zhang
View Article