Tested Applications
| Positive WB detected in | mouse kidney tissue, HEK-293 cells, mouse colon tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
16699-1-AP targets NKD2 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NKD2 fusion protein Ag10094 Predict reactive species |
| Full Name | naked cuticle homolog 2 (Drosophila) |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC012176 |
| Gene Symbol | NKD2 |
| Gene ID (NCBI) | 85409 |
| RRID | AB_2151468 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969F2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NKD2 antibody 16699-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene NKD2, a negative regulator of Wnt signaling, suppresses tumor growth and metastasis in osteosarcoma.
| ||
Biosci Biotechnol Biochem Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells. | ||
Kaohsiung J Med Sci Curcumin suppresses the Wnt/β-catenin signaling pathway by inhibiting NKD2 methylation to ameliorate intestinal ischemia/reperfusion injury | ||
Rejuvenation Res Tripartite Motif-Containing Protein 65 Promotes Proliferation and Inhibits Ferroptosis in Prostate Cancer via Enhancing NKD Inhibitor of WNT Signaling Pathway 2 Ubiquitination |
