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PITPNA Polyclonal antibody

PITPNA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16613-1-AP

Synonyms

PI TP alpha, PITPN, PITPNA, PtdIns transfer protein alpha, PtdInsTP alpha, VIB1A

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PITPNA Polyclonal antibody (16613-1-AP)

    WB analysis of HEK-293 using 16613-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16613-1-AP (PITPNA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PITPNA Polyclonal antibody (16613-1-AP)

    WB analysis of human brain using 16613-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16613-1-AP (PITPNA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PITPNA Polyclonal antibody (16613-1-AP)

    IP experiment of HEK-293 using 16613-1-AP

    IP result of anti-PITPNA (IP:16613-1-AP, 4ug; Detection:16613-1-AP 1:500) with HEK-293 cells lysate 1520ug.

  • Immunohistochemistry (IHC) staining of human lung tissue using PITPNA Polyclonal antibody (16613-1-AP)

    IHC staining of human lung using 16613-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16613-1-AP (PITPNA antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PITPNA Polyclonal antibody (16613-1-AP)

    IHC staining of human lung using 16613-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16613-1-AP (PITPNA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PITPNA Polyclonal antibody (16613-1-AP)

    IHC staining of human heart using 16613-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16613-1-AP (PITPNA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PITPNA Polyclonal antibody (16613-1-AP)

    IHC staining of human heart using 16613-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16613-1-AP (PITPNA antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PITPNA Polyclonal antibody (16613-1-AP)

    IHC staining of human brain using 16613-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16613-1-AP (PITPNA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PITPNA Polyclonal antibody (16613-1-AP)

    IHC staining of human brain using 16613-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16613-1-AP (PITPNA antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung tissue, human brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16613-1-AP targets PITPNA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PITPNA fusion protein Ag9849
Full Name phosphatidylinositol transfer protein, alpha
Calculated Molecular Weight 270 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC045108
Gene Symbol PITPNA
Gene ID (NCBI) 5306
RRIDAB_2299692
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PITPNA antibody 16613-1-APDownload protocol
IHC protocol for PITPNA antibody 16613-1-APDownload protocol
IP protocol for PITPNA antibody 16613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Dev Cell

A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis.

Authors - Zhigang Xie
View Article
ratWB

BMC Complement Altern Med

Differential proteomics analysis of the analgesic effect of electroacupuncture intervention in the hippocampus following neuropathic pain in rats.

Authors - Gao Yong-Hui YH
View Article
mouseWB,IF

Nat Commun

Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction

Authors - Yu-Te Yeh
View Article