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SMAD9 Polyclonal antibody

SMAD9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16397-1-AP

Synonyms

MAD homolog 9, MADH6, MADH9, Mothers against DPP homolog 9, SMAD 9, SMAD family member 9, SMAD8A, SMAD8B, SMAD9

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using SMAD9 Polyclonal antibody (16397-1-AP)

    WB analysis of HeLa using 16397-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using SMAD9 Polyclonal antibody (16397-1-AP)

    WB analysis of mouse lung using 16397-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SMAD9 Polyclonal antibody (16397-1-AP)

    WB analysis of A549 using 16397-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using SMAD9 Polyclonal antibody (16397-1-AP)

    WB analysis of mouse lung using 16397-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SMAD9 Polyclonal antibody (16397-1-AP)

    WB analysis of HEK-293 using 16397-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SMAD9 Polyclonal antibody (16397-1-AP)

    WB analysis of HepG2 using 16397-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SMAD9 Polyclonal antibody (16397-1-AP)

    WB analysis of HeLa using 16397-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SMAD9 Polyclonal antibody (16397-1-AP)

    WB analysis of A549 using 16397-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, HepG2 cells, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16397-1-AP targets SMAD9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD9 fusion protein Ag9490
Full Name SMAD family member 9
Calculated Molecular Weight 430 aa, 49 kDa
Observed Molecular Weight 49-55 kDa
GenBank Accession NumberBC011559
Gene Symbol SMAD9
Gene ID (NCBI) 4093
RRIDAB_2270847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SMAD9 antibody 16397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

IUBMB Life

LncRNA RP11-301G19.1 is required for the maintenance of vascular smooth muscle cell contractile phenotype via sponging miR-17-5P/ATOH8 axis

Authors - Shuning Hao
View Article
mouseWB

Sci Rep

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.

Authors - Xueni Wang
View Article
mouseWB

Cell Death Discov

Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling

Authors - Ping Yin
View Article