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GINS3 Polyclonal antibody

GINS3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15651-1-AP

Synonyms

GINS complex subunit 3, GINS3, PSF3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using GINS3 Polyclonal antibody (15651-1-AP)

    WB analysis of Jurkat using 15651-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15651-1-AP (GINS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using GINS3 Polyclonal antibody (15651-1-AP)

    WB analysis of NIH/3T3 using 15651-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15651-1-AP (GINS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using GINS3 Polyclonal antibody (15651-1-AP)

    IP experiment of Jurkat using 15651-1-AP

    IP result of anti-GINS3 (IP:15651-1-AP, 4ug; Detection:15651-1-AP 1:300) with Jurkat cells lysate 3400ug.

Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

15651-1-AP targets GINS3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GINS3 fusion protein Ag8247
Full Name GINS complex subunit 3 (Psf3 homolog)
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC005879
Gene Symbol GINS3
Gene ID (NCBI) 64785
RRIDAB_2247477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GINS3 antibody 15651-1-APDownload protocol
IP protocol for GINS3 antibody 15651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis.

Authors - Roberto Bellelli
View Article
humanWB

J Clin Invest

Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency.

Authors - Julien Cottineau
View Article
humanWB

JCI Insight

Partial loss-of-function mutations in GINS4 lead to NK cell deficiency with neutropenia

Authors - Matilde I. Conte
View Article
humanWB

Cell Rep

Loss of POLE3-POLE4 unleashes replicative gap accumulation upon treatment with PARP inhibitors

Authors - Bethany Rebekah Hill
View Article