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  • Featured Product
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DHFR Polyclonal antibody

DHFR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15194-1-AP

Synonyms

EC:1.5.1.3, DHFRP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of HeLa using 15194-1-AP

    WB result of DHFR antibody (15194-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis using 15194-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of Jurkat using 15194-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of K-562 using 15194-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of HeLa using 15194-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using DHFR Polyclonal antibody (15194-1-AP)

    WB analysis of Raji using 15194-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using DHFR Polyclonal antibody (15194-1-AP)

    IP experiment of Raji using 15194-1-AP

    IP result of anti-DHFR (IP:15194-1-AP, 3ug; Detection:15194-1-AP 1:500) with Raji cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human liver cancer using 15194-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human liver cancer using 15194-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human breast cancer using 15194-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human breast cancer using 15194-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human liver cancer using 15194-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Polyclonal antibody (15194-1-AP)

    IHC staining of human liver cancer using 15194-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHFR Polyclonal antibody (15194-1-AP)

    IF Staining of HeLa using 15194-1-AP

    Immunofluorescent analysis of HeLa cells using 15194-1-AP (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15194-1-AP targets DHFR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHFR fusion protein Ag7331
Full Name dihydrofolate reductase
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC000192
Gene Symbol DHFR
Gene ID (NCBI) 1719
RRIDAB_2091587
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

Protocols

Product Specific Protocols
WB protocol for DHFR antibody 15194-1-APDownload protocol
IHC protocol for DHFR antibody 15194-1-APDownload protocol
IF protocol for DHFR antibody 15194-1-APDownload protocol
IP protocol for DHFR antibody 15194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
View Article
humanWB

Am J Hum Genet

Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency.

Authors - Banka Siddharth S
View Article
mouseWB

J Exp Med

CXCR4 regulates Plasmodium development in mouse and human hepatocytes.

Authors - Hironori Bando
View Article
mouseWB

Food Funct

The protective effect of polyunsaturated fatty acid intake during pregnancy against embryotoxicity of sodium valproate in mice

Authors - Kelei Li
View Article
humanWB

Cancer Sci

Thymidylate synthase inhibitor raltitrexed can induce high levels of DNA damage in MYCN-amplified neuroblastoma cells.

Authors - Ken Yamashita
View Article
WB

Structure

Disease-linked mutations cause exposure of a protein quality control degron.

Authors - Caroline Kampmeyer
View Article