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ALG2 Polyclonal antibody

ALG2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15092-1-AP

Synonyms

ALG2, CDGIi, hALPG2, NET38

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ALG2 Polyclonal antibody (15092-1-AP)

    WB analysis using 15092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15092-1-AP (ALG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ALG2 Polyclonal antibody (15092-1-AP)

    IHC staining of human pancreas cancer using 15092-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15092-1-AP (ALG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ALG2 Polyclonal antibody (15092-1-AP)

    IHC staining of human pancreas cancer using 15092-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15092-1-AP (ALG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15092-1-AP targets ALG2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALG2 fusion protein Ag4833
Full Name asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 47 kDa, 37 kDa
GenBank Accession NumberBC015126
Gene Symbol ALG2
Gene ID (NCBI) 85365
RRIDAB_2878108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ALG2 antibody 15092-1-APDownload protocol
IHC protocol for ALG2 antibody 15092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

A mechanism that transduces lysosomal damage signals to stress granule formation for cell survival

Authors - Jacob Duran
View Article