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AP1S2 Polyclonal antibody

AP1S2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15043-1-AP

Synonyms

AP 1 complex subunit sigma 2, AP1S2, DC22, MRX59, Sigma adaptin 1B, SIGMA1B, Sigma1B adaptin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP1S2 Polyclonal antibody (15043-1-AP)

    WB analysis using 15043-1-AP

    rat epididymis tissue was subjected to SDS PAGE followed by western blot with 15043-1-AP (AP1S2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inrat epididymis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15043-1-AP targets AP1S2 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP1S2 fusion protein Ag6911
Full Name adaptor-related protein complex 1, sigma 2 subunit
Calculated Molecular Weight 19 kDa
Observed Molecular Weight19-24 kDa
GenBank Accession NumberBC001117
Gene Symbol AP1S2
Gene ID (NCBI) 8905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP1S2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes small subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

Protocols

Product Specific Protocols
WB protocol for AP1S2 antibody 15043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols