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WIBG Polyclonal antibody

WIBG Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14658-1-AP

Synonyms

PYM1, PYM homolog 1 exon junction complex-associated factor, PYM, Protein wibg homolog, Partner of Y14 and mago

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse colon tissue using WIBG Polyclonal antibody (14658-1-AP)

    IHC staining of mouse colon using 14658-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14658-1-AP (WIBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using WIBG Polyclonal antibody (14658-1-AP)

    IF Staining of U2OS using 14658-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using WIBG antibody (14658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14658-1-AP targets WIBG in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WIBG fusion protein Ag6276
Full Name within bgcn homolog (Drosophila)
Calculated Molecular Weight 23 kDa
GenBank Accession NumberBC006135
Gene Symbol WIBG
Gene ID (NCBI) 84305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).

Protocols

Product Specific Protocols
IHC protocol for WIBG antibody 14658-1-APDownload protocol
IF protocol for WIBG antibody 14658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols