VGAT Polyclonal antibody
VGAT Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 14471-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | unboiled mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
Product Information
14471-1-AP targets VGAT in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, caenorhabditis elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VGAT fusion protein Ag5843 |
| Full Name | solute carrier family 32 (GABA vesicular transporter), member 1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC053582 |
| Gene Symbol | VGAT |
| Gene ID (NCBI) | 140679 |
| RRID | AB_10644324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VGAT antibody 14471-1-AP | Download protocol |
| IHC protocol for VGAT antibody 14471-1-AP | Download protocol |
| IF protocol for VGAT antibody 14471-1-AP | Download protocol |
| IP protocol for VGAT antibody 14471-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Commun Mutation-induced loss of APP function causes GABAergic depletion in recessive familial Alzheimer's disease: analysis of Osaka mutation-knockin mice. | ||
PLoS One Mutant TDP-43 and FUS cause age-dependent paralysis and neurodegeneration in C. elegans. | ||
Curr Issues Mol Biol miR-92a-2-5p Regulates the Proliferation and Differentiation of ASD-Derived Neural Progenitor Cells. | ||
Biol Trace Elem Res Exercise Ameliorates Fluoride-induced Anxiety- and Depression-like Behavior in Mice: Role of GABA. | ||
Exp Anim TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Reyes (Verified Customer) (03-04-2024) | vGAT worked on my human brain FFPE sections although the quantification would be difficult due to the high autofluorescence
 |
FH Mandi (Verified Customer) (03-10-2020) | Did not stain the nerve terminals well. Signal was localized to the soma mainly.
|
FH Ute (Verified Customer) (03-03-2020) | blocking solution 5% skimmed milk in 1x TBS-T
|
