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TFIP11 Polyclonal antibody

TFIP11 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14436-1-AP

Synonyms

STIP, Septin and tuftelin-interacting protein 1, NTR1, HSPC006, hNtr1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TFIP11 Polyclonal antibody (14436-1-AP)

    WB analysis of HeLa using 14436-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TFIP11 Polyclonal antibody (14436-1-AP)

    WB analysis of K-562 using 14436-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TFIP11 Polyclonal antibody (14436-1-AP)

    WB analysis of MCF-7 using 14436-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TFIP11 Polyclonal antibody (14436-1-AP)

    WB analysis of mouse testis using 14436-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TFIP11 Polyclonal antibody (14436-1-AP)

    IF Staining of HeLa using 14436-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14436-1-AP (TFIP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14436-1-AP targets TFIP11 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFIP11 fusion protein Ag5821
Full Name tuftelin interacting protein 11
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC033080
Gene Symbol TFIP11
Gene ID (NCBI) 24144
RRIDAB_11182269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.

Protocols

Product Specific Protocols
WB protocol for TFIP11 antibody 14436-1-APDownload protocol
IF protocol for TFIP11 antibody 14436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols