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ABT1 Polyclonal antibody

ABT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14148-1-AP

Synonyms

hABT1, Esf2, Activator of basal transcription 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ABT1 Polyclonal antibody (14148-1-AP)

    WB analysis of mouse liver using 14148-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14148-1-AP (ABT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ABT1 Polyclonal antibody (14148-1-AP)

    WB analysis of HeLa using 14148-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14148-1-AP (ABT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using ABT1 Polyclonal antibody (14148-1-AP)

    IHC staining of human brain using 14148-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14148-1-AP (ABT1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ABT1 Polyclonal antibody (14148-1-AP)

    IHC staining of human brain using 14148-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14148-1-AP (ABT1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABT1 Polyclonal antibody (14148-1-AP)

    IF Staining of HeLa using 14148-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14148-1-AP (ABT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14148-1-AP targets ABT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABT1 fusion protein Ag5317
Full Name activator of basal transcription 1
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31-32 kDa
GenBank Accession NumberBC066313
Gene Symbol ABT1
Gene ID (NCBI) 29777
RRIDAB_10640440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABT1 antibody 14148-1-APDownload protocol
IHC protocol for ABT1 antibody 14148-1-APDownload protocol
IF protocol for ABT1 antibody 14148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

JCI Insight

ABT1 modifies SMARD1 pathology via interactions with IGHMBP2 and stimulation of ATPase and helicase activity

Authors - Gangadhar P Vadla
View Article