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NISCH Polyclonal antibody

NISCH Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 13813-1-AP

Synonyms

I1R, I-1 receptor candidate protein, I-1, I 1 receptor candidate protein, I 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NISCH Polyclonal antibody (13813-1-AP)

    WB analysis using 13813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13813-1-AP (NISCH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using NISCH Polyclonal antibody (13813-1-AP)

    IP experiment of Jurkat using 13813-1-AP

    IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with Jurkat cells lysate 1560 ug.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NISCH Polyclonal antibody (13813-1-AP)

    IP experiment of mouse brain using 13813-1-AP

    IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NISCH Polyclonal antibody (13813-1-AP)

    IHC staining of human stomach using 13813-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13813-1-AP (NISCH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13813-1-AP targets NISCH in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NISCH fusion protein Ag4880
Full Name nischarin
Calculated Molecular Weight 1504 aa, 167 kDa
Observed Molecular Weight 190-200 kDa
GenBank Accession NumberBC038102
Gene Symbol NISCH
Gene ID (NCBI) 11188
RRIDAB_10693526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NISCH antibody 13813-1-APDownload protocol
IHC protocol for NISCH antibody 13813-1-APDownload protocol
IP protocol for NISCH antibody 13813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Anesth Analg

Dexmedetomidine Ameliorates Perioperative Neurocognitive Disorders by Suppressing Monocyte-Derived Macrophages in Mice With Preexisting Traumatic Brain Injury.

Authors - Natsumi Kii
View Article
humanWB,IF

Biochem Biophys Res Commun

Expression of Nischarin negatively correlates with estrogen receptor and alters apoptosis, migration and invasion in human breast cancer.

Authors - Chan Chang
View Article