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  • KD/KO Validated

Carboxypeptidase E Polyclonal antibody

Carboxypeptidase E Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 13710-1-AP

Synonyms

Prohormone-processing carboxypeptidase, EC:3.4.17.10, CPH, CPE, Carboxypeptidase H



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13710-1-AP targets Carboxypeptidase E in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase E fusion protein Ag4647
Full Name carboxypeptidase E
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 50 kDa, 52 kDa
GenBank Accession NumberBC033866
Gene Symbol Carboxypeptidase E
Gene ID (NCBI) 1363
RRIDAB_2083913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IHC protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IF protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
IP protocol for Carboxypeptidase E antibody 13710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Metab

Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion.

Authors - Guo-Fang Zhang
mouseWB

Diabetes

Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Authors - Seokwon Jo

Am J Physiol Endocrinol Metab

Loss of the Golgi-localized v-ATPase subunit does not alter insulin granule formation or pancreatic islet β-cell function

Authors - Cierra K Boyer
human, mouseIHC

Brain Pathol

Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.

Authors - Virginia Plá
humanWB,IHC

Front Genet

Development and Validation of a Novel Gene Signature for Predicting the Prognosis by Identifying m5C Modification Subtypes of Cervical Cancer.

Authors - Jing Yu
  • KD Validated
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li