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Carboxypeptidase E Polyclonal antibody
Carboxypeptidase E Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 13710-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human pituitary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 4 publications below |
IF | See 3 publications below |
Product Information
13710-1-AP targets Carboxypeptidase E in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Carboxypeptidase E fusion protein Ag4647 |
Full Name | carboxypeptidase E |
Calculated Molecular Weight | 476 aa, 53 kDa |
Observed Molecular Weight | 50 kDa, 52 kDa |
GenBank Accession Number | BC033866 |
Gene Symbol | Carboxypeptidase E |
Gene ID (NCBI) | 1363 |
RRID | AB_2083913 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.
Protocols
Product Specific Protocols | |
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WB protocol for Carboxypeptidase E antibody 13710-1-AP | Download protocol |
IHC protocol for Carboxypeptidase E antibody 13710-1-AP | Download protocol |
IF protocol for Carboxypeptidase E antibody 13710-1-AP | Download protocol |
IP protocol for Carboxypeptidase E antibody 13710-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion. | ||
Diabetes Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function. | ||
Am J Physiol Endocrinol Metab Loss of the Golgi-localized v-ATPase subunit does not alter insulin granule formation or pancreatic islet β-cell function | ||
Brain Pathol Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease. | ||
Front Genet Development and Validation of a Novel Gene Signature for Predicting the Prognosis by Identifying m5C Modification Subtypes of Cervical Cancer.
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