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NR2C1 Polyclonal antibody

NR2C1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13658-1-AP

Synonyms

NR2C1, Orphan nuclear receptor TR2, Testicular receptor 2, TR2

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NR2C1 Polyclonal antibody (13658-1-AP)

    WB analysis of SH-SY5Y using 13658-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13658-1-AP (NR2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NR2C1 Polyclonal antibody (13658-1-AP)

    WB analysis of PC-3 using 13658-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13658-1-AP (NR2C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NR2C1 Polyclonal antibody (13658-1-AP)

    IHC staining of human prostate cancer using 13658-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NR2C1 Polyclonal antibody (13658-1-AP)

    IHC staining of human prostate cancer using 13658-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

Tested Applications

Positive WB detected inSH-SY5Y cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13658-1-AP targets NR2C1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR2C1 fusion protein Ag4554
Full Name nuclear receptor subfamily 2, group C, member 1
Calculated Molecular Weight 467 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC040141
Gene Symbol NR2C1
Gene ID (NCBI) 7181
RRIDAB_2236290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptor subfamily 2 group C member 1 (NR2C1), also known as Orphan nuclear receptor TR2, is a member of the steroid/thyroid hormone receptor superfamily that controls a variety of processes, including growth, differentiation and development. TR2 is known to bind to regulatory elements of the erythropoietin gene, the muscle-specific aldolase A gene and the CNTF-15 gene. In addition to TR2, a related orphan receptor, designated TR4, has been identified. TR4 forms heterodimers with TR2, which are thought to be involved in neurogenesis and germ cell development. TR2 is known to be downregulated by both p53 and ionizing radiation, and it may play a role in linking p53 to members of the steroid recepr family.

Protocols

Product Specific Protocols
WB protocol for NR2C1 antibody 13658-1-APDownload protocol
IHC protocol for NR2C1 antibody 13658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols