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  • Featured Product
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PPEF1 Polyclonal antibody

PPEF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12875-1-AP

Synonyms

PP7, PPEF, PPEF 1, PPEF1, PPP7C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPEF1 Polyclonal antibody (12875-1-AP)

    WB analysis using 12875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12875-1-AP (PPEF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PPEF1 Polyclonal antibody (12875-1-AP)

    WB analysis of HeLa using 12875-1-AP

    WB result of PPEF1 antibody (12875-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPEF1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PPEF1 Polyclonal antibody (12875-1-AP)

    IHC staining of mouse testis using 12875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PPEF1 Polyclonal antibody (12875-1-AP)

    IHC staining of mouse testis using 12875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, PC-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12875-1-AP targets PPEF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPEF1 fusion protein Ag3553
Full Name protein phosphatase, EF-hand calcium binding domain 1
Calculated Molecular Weight 653 aa, 76 kDa
Observed Molecular Weight 69-76 kDa
GenBank Accession NumberBC036026
Gene Symbol PPEF1
Gene ID (NCBI) 5475
RRIDAB_2166375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPEF1 antibody 12875-1-APDownload protocol
IHC protocol for PPEF1 antibody 12875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols