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MOBKL1A Polyclonal antibody

MOBKL1A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12790-1-AP

Synonyms

MATS2, Mob1 homolog 1A, Mob1A, Mob1B, MOB4A, MOBKL1A, Protein Mob4A

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using MOBKL1A Polyclonal antibody (12790-1-AP)

    WB analysis of Jurkat using 12790-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12790-1-AP (MOBKL1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using MOBKL1A Polyclonal antibody (12790-1-AP)

    IP experiment of Jurkat using 12790-1-AP

    IP result of anti-MOBKL1A (IP:12790-1-AP, 4ug; Detection:12790-1-AP 1:1000) with Jurkat cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MOBKL1A Polyclonal antibody (12790-1-AP)

    IHC staining of human pancreas cancer using 12790-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12790-1-AP (MOBKL1A antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12790-1-AP targets MOBKL1A in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOBKL1A fusion protein Ag3509
Full Name MOB1, Mps One Binder kinase activator-like 1A (yeast)
Calculated Molecular Weight 216 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC038112
Gene Symbol MOBKL1A
Gene ID (NCBI) 92597
RRIDAB_2266566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MOBKL1A antibody 12790-1-APDownload protocol
IHC protocol for MOBKL1A antibody 12790-1-APDownload protocol
IP protocol for MOBKL1A antibody 12790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Breast Cancer Res

Circular RNA circCCDC85A inhibits breast cancer progression via acting as a miR-550a-5p sponge to enhance MOB1A expression

Authors - Lingjiao Meng
View Article
mouseIHC

Biol Reprod

Lats1 Deletion Causes Increased Germ Cell Apoptosis and Follicular Cysts in Mouse Ovaries.

Authors - Tianyanxin Sun
View Article
humanWB

Biochem Genet

Growth Inhibition Accompanied by MOB1 Upregulation in Human Acute Lymphoid Leukemia Cells by 3-Deazaneplanocin A.

Authors - Jianzhen Shen
View Article
humanIHC

Cell Death Dis

Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway.

Authors - Yang Han
View Article