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LIN7B Polyclonal antibody

LIN7B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12732-1-AP

Synonyms

hLin7B, hVeli2, lin 7 homolog B (C. elegans), LIN 7B, LIN7B, MALS 2, MALS2, Mammalian lin seven protein 2, Protein lin 7 homolog B, Veli 2, VELI2, Vertebrate lin 7 homolog 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using LIN7B Polyclonal antibody (12732-1-AP)

    WB analysis of human heart using 12732-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12732-1-AP (LIN7B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using LIN7B Polyclonal antibody (12732-1-AP)

    WB analysis of K-562 using 12732-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12732-1-AP (LIN7B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using LIN7B Polyclonal antibody (12732-1-AP)

    WB analysis of A549 using 12732-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12732-1-AP (LIN7B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using LIN7B Polyclonal antibody (12732-1-AP)

    WB analysis of human brain using 12732-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12732-1-AP (LIN7B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman heart tissue, A549 cells, human brain tissue, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12732-1-AP targets LIN7B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN7B fusion protein Ag3437
Full Name lin-7 homolog B (C. elegans)
Calculated Molecular Weight 207 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC027618
Gene Symbol LIN7B
Gene ID (NCBI) 64130
RRIDAB_2234548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LIN7B antibody 12732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols