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- KD/KO Validated
COQ6 Polyclonal antibody
COQ6 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12481-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse ovary tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
12481-1-AP targets COQ6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COQ6 fusion protein Ag3164 |
| Full Name | coenzyme Q6 homolog, monooxygenase (S. cerevisiae) |
| Calculated Molecular Weight | 468 aa, 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC014181 |
| Gene Symbol | COQ6 |
| Gene ID (NCBI) | 51004 |
| RRID | AB_10644143 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COQ6(Coenzyme Q10 monooxygenase 6) is also named CGI-10 and belongs to the ubiH/COQ6 family. It is an evolutionarily conserved flavin-dependent monooxygenase required for the biosynthesis of coenzyme Q10 or ubiquinone. It has 3 isoforms with a molecular mass of 51 kDa, 43 kDa and 49 kDa. In the inner ear, Coq6 is expressed in the spiral ganglion, stria vascularis cells, and spiral ligament(PMID:21540551 ). Defects in COQ6 are the cause of coenzyme Q10 deficiency, primary, type 6 (COQ10D6).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COQ6 antibody 12481-1-AP | Download protocol |
| IHC protocol for COQ6 antibody 12481-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Mol Med β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice. | ||
Aging Cell Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging. | ||
Oxid Med Cell Longev Vanillic Acid Restores Coenzyme Q Biosynthesis and ATP Production in Human Cells Lacking COQ6.
| ||
Chin Med J (Engl) New Mutation of Coenzyme Q10 Monooxygenase 6 Causing Podocyte Injury in a Focal Segmental Glomerulosclerosis Patient. | ||
Antioxidants (Basel) Co-Enzyme Q10 Supplementation Rescues Cumulus Cells Dysfunction in a Maternal Aging Model. |
