- Featured Product
- KD/KO Validated
FAM107A Polyclonal antibody
FAM107A Polyclonal Antibody for IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 12176-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
12176-1-AP targets FAM107A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAM107A fusion protein Ag2820 |
| Full Name | family with sequence similarity 107, member A |
| Calculated Molecular Weight | 144 aa, 17 kDa |
| GenBank Accession Number | BC010561 |
| Gene Symbol | FAM107A |
| Gene ID (NCBI) | 11170 |
| RRID | AB_2877832 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FAM107A, also named as TU3A, encodes a 144 amino-acid protein, which contains a coiled-coil domain and a nuclear localization signal. FAM107A as a transcription factor regulates some gene transcription and signal transduction in cell. With the exception of peripheral blood cells, FAM107A is widely expressed in normal tissues, but shows significant loss of expression in renal cell carcinomas and frequent loss of expression in cervical, gastric, ovarian and non small cell lung cancers. Transfection of FAM107A mRNA into cancer cell line inhibits cell growth and proliferation, supporting the evidence that the gene functions as an important tumor suppressor.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM107A antibody 12176-1-AP | Download protocol |
| IF protocol for FAM107A antibody 12176-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet A severe mouse model of spinal muscular atrophy develops early systemic inflammation. | ||
Arch Biochem Biophys Loss of family with sequence similarity 107, member A (FAM107A) induces browning in 3T3-L1 adipocytes.
| ||
Cancers (Basel) FAM107A Inactivation Associated with Promoter Methylation Affects Prostate Cancer Progression through the FAK/PI3K/AKT Pathway | ||
Pathol Res Pract FAM107A as a tumor suppressor in esophageal squamous carcinoma inhibits growth and metastasis |
