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  • Featured Product
  • KD/KO Validated

PTS Polyclonal antibody

PTS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12150-1-AP

Synonyms

PTPS, PTP synthase, EC:4.2.3.12, 6-pyruvoyl tetrahydrobiopterin synthase

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using PTS Polyclonal antibody (12150-1-AP)

    WB analysis of human brain using 12150-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12150-1-AP (PTS antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PTS Polyclonal antibody (12150-1-AP)

    IHC staining of human gliomas using 12150-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12150-1-AP (PTS antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTS Polyclonal antibody (12150-1-AP)

    IF Staining of HeLa using 12150-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12150-1-AP (PTS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12150-1-AP targets PTS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTS fusion protein Ag2798
Full Name 6-pyruvoyltetrahydropterin synthase
Calculated Molecular Weight 145 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC009686
Gene Symbol PTS
Gene ID (NCBI) 5805
RRIDAB_2173398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PTS antibody 12150-1-APDownload protocol
IHC protocol for PTS antibody 12150-1-APDownload protocol
IF protocol for PTS antibody 12150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia.

Authors - Qin Zhao
View Article
mouseWB

Acta Physiol (Oxf)

Tetrahydrobiopterin synthesis and metabolism is impaired in dystrophin-deficient mdx mice and humans.

Authors - Angus Lindsay
View Article
humanWB,IHC

Transl Lung Cancer Res

PTS is activated by ATF4 and promotes lung adenocarcinoma development via the Wnt pathway

Authors - Wei Ma
  • KD Validated
View Article