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- KD/KO Validated
PBXIP1 Polyclonal antibody
PBXIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 12102-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, A549 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 10 publications below |
| IHC | See 5 publications below |
| IF | See 3 publications below |
| ChIP | See 1 publications below |
Product Information
12102-1-AP targets PBXIP1 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PBXIP1 fusion protein Ag2746 |
| Full Name | pre-B-cell leukemia homeobox interacting protein 1 |
| Calculated Molecular Weight | 731 aa, 81 kDa |
| Observed Molecular Weight | 95-100 kDa |
| GenBank Accession Number | BC016852 |
| Gene Symbol | PBXIP1 |
| Gene ID (NCBI) | 57326 |
| RRID | AB_2160484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PBXIP1 (pre-B-cell leukemia transcription factor-interacting protein 1), also known as HPIP (hematopoietic PBX-interacting protein), is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. PBXIP1 is a nucleo-cytoplasmic shuttling protein, mainly localized in the cytosol and in small amounts in the nucleus (PMID: 10825160). It interacts with PBX1 as well as PBX2 and PBX3. PBXIP1 inhibits the ability of PBX-HOX heterodimers to bind to target sequences and strongly inhibits the transactivation activity of E2A-PBX. PBXIP1 is also reported to be a microtubule-binding protein, which regulates estrogen receptor functions and plays a role in cancer development and progression (PMID: 17043237; 18302941; 23321675). The predicted PBXIP1 protein has a calculated molecular mass of 81 kDa. This polyclonal antibody raised against 1-300aa of human PBXIP1 recognizes endogenous PBXIP1 with an apparent molecular weight of 95-100 kDa. The slow migration of PBXIP1 possibly results from either post-translational modifications or intrinsic SDS-resistant folding of the protein (PMID: 10825160; 17043237).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PBXIP1 antibody 12102-1-AP | Download protocol |
| IHC protocol for PBXIP1 antibody 12102-1-AP | Download protocol |
| IF protocol for PBXIP1 antibody 12102-1-AP | Download protocol |
| IP protocol for PBXIP1 antibody 12102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.
| ||
Cell Death Differ MDM2 restrains estrogen-mediated AKT activation by promoting TBK1-dependent HPIP degradation. | ||
Oncogenesis The interplay between HPIP and casein kinase 1α promotes renal cell carcinoma growth and metastasis via activation of mTOR pathway.
| ||
Cancer Sci Hematopoietic pre-B cell leukemia transcription factor interacting protein is overexpressed in gastric cancer and promotes gastric cancer cell proliferation, migration, and invasion.
| ||
Sci Rep HPIP is upregulated in colorectal cancer and regulates colorectal cancer cell proliferation, apoptosis and invasion. | ||
IUBMB Life HPIP is upregulated in liver cancer and promotes hepatoma cell proliferation via activation of G2/M transition.
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