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  • Featured Product
  • KD/KO Validated

TTC1 Polyclonal antibody

TTC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11676-1-AP

Synonyms

TPR1, TPR repeat protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TTC1 Polyclonal antibody (11676-1-AP)

    WB analysis of HEK-293 using 11676-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TTC1 Polyclonal antibody (11676-1-AP)

    WB analysis using 11676-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TTC1 Polyclonal antibody (11676-1-AP)

    WB analysis using 11676-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TTC1 Polyclonal antibody (11676-1-AP)

    WB analysis of HepG2 using 11676-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TTC1 Polyclonal antibody (11676-1-AP)

    WB analysis of Jurkat using 11676-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TTC1 Polyclonal antibody (11676-1-AP)

    IHC staining of human colon cancer using 11676-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11676-1-AP (TTC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TTC1 Polyclonal antibody (11676-1-AP)

    IF Staining of HepG2 using 11676-1-AP

    Immunofluorescent analysis of HepG2 cells using 11676-1-AP (TTC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TTC1 Polyclonal antibody (11676-1-AP)

    IF Staining of HepG2 using 11676-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TTC1 antibody (11676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11676-1-AP targets TTC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC1 fusion protein Ag2277
Full Name tetratricopeptide repeat domain 1
Calculated Molecular Weight33.5 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000942
Gene Symbol TTC1
Gene ID (NCBI) 7265
RRIDAB_11182936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TTC1 antibody 11676-1-APDownload protocol
IHC protocol for TTC1 antibody 11676-1-APDownload protocol
IF protocol for TTC1 antibody 11676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Mol Cell

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

Authors - Gayathri Muthukumar
  • KD Validated
View Article