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  • Featured Product
  • KD/KO Validated

DDX20 Polyclonal antibody

DDX20 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 11324-1-AP

Synonyms

Gemin 3, EC:3.6.1.15, DP103, DEAD box protein DP 103, DEAD box protein 20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX20 Polyclonal antibody (11324-1-AP)

    WB analysis using 11324-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DDX20 Polyclonal antibody (11324-1-AP)

    WB analysis of mouse testis using 11324-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DDX20 Polyclonal antibody (11324-1-AP)

    WB analysis of Jurkat using 11324-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DDX20 Polyclonal antibody (11324-1-AP)

    IP experiment of HeLa using 11324-1-AP

    IP result of anti-DDX20 (IP:11324-1-AP, 4ug; Detection:11324-1-AP 1:2000) with HeLa cells lysate 1880 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX20 Polyclonal antibody (11324-1-AP)

    IHC staining of human breast cancer using 11324-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11324-1-AP (DDX20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX20 Polyclonal antibody (11324-1-AP)

    IHC staining of human breast cancer using 11324-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11324-1-AP (DDX20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDX20 Polyclonal antibody (11324-1-AP)

    IHC staining of human colon cancer using 11324-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11324-1-AP (DDX20 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX20 Polyclonal antibody (11324-1-AP)

    IF Staining of HepG2 using 11324-1-AP

    Immunofluorescent analysis of HepG2 cells, using DDX20 antibody 11324-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX20 Polyclonal antibody (11324-1-AP)

    IF Staining of HeLa using 11324-1-AP

    Immunofluorescent analysis of Hela cells, using DDX20 antibody 11324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using DDX20 Polyclonal antibody (11324-1-AP)

    FC experiment of HepG2 using 11324-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DDX20 (11324-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, mouse testis tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11324-1-AP targets DDX20 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX20 fusion protein Ag1863
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Calculated Molecular Weight 824 aa, 92 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC011556
Gene Symbol DDX20
Gene ID (NCBI) 11218
RRIDAB_2092401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (DDX20), also known as DP103 or Gemin3, is a member of the DEAD box protein family expressed ubiquitously. DEAD family proteins use energy from ATP hydrolysis for RNA chaperoning and RNPase activity (PMID: 27121695). As a core member of the survival motor neuron (SMN) complex, DDX20 participate in small nuclear ribonucleoprotein (snRNP) biogenesis. Second, DDX20 have direct roles in gene expression in view of its implication in transcription and post-transcriptional gene silencing. Addition, the false expression of DDX20 could have deleterious effects on cellular homeostasis thus leading to cancer development and progression (PMID:29523774). Anymore, DDX20 could be identified as a biomarker and metastasis-driving oncogene of human breast cancer (PMID: 25083991). The detected weight of DDX20 is slightly higher than the theoretical molecular weight that is because of phosphorylation after translation.

Protocols

Product Specific Protocols
WB protocol for DDX20 antibody 11324-1-APDownload protocol
IHC protocol for DDX20 antibody 11324-1-APDownload protocol
IF protocol for DDX20 antibody 11324-1-APDownload protocol
IP protocol for DDX20 antibody 11324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

J Clin Invest

DEAD-box helicase DP103 defines metastatic potential of human breast cancers.

Authors - Eun Myoung Shin
  • KD Validated
View Article
mouseWB,IHC,IF

Dev Cell

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

Authors - Dingfeng Zou
View Article
humanIHC

Aging (Albany NY)

An artificial neural network model to diagnose non-obstructive azoospermia based on RNA-binding protein-related genes

Authors - Fan Peng
View Article
humanWB

Comput Struct Biotechnol J

Analysis of the expression, function, prognosis and co-expression genes of DDX20 in gastric cancer.

Authors - Qingshui Wang
View Article
humanIHC

Arthritis Res Ther

Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies.

Authors - Ping Li
View Article
WB

Biosci Rep

FOXL2 Down-regulates Vitellogenin Expression at Mature Stage in Eriocheir sinensis.

Authors - Qing Li
View Article