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EIF2B3 Polyclonal antibody

EIF2B3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11296-2-AP

Synonyms

Translation initiation factor eIF2B subunit gamma, EIF2Bgamma, eIF2B GDP-GTP exchange factor subunit gamma, EIF 2B

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EIF2B3 Polyclonal antibody (11296-2-AP)

    WB analysis using 11296-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11296-2-AP (EIF2B3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EIF2B3 Polyclonal antibody (11296-2-AP)

    IHC staining of mouse colon using 11296-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11296-2-AP (EIF2B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EIF2B3 Polyclonal antibody (11296-2-AP)

    IHC staining of mouse colon using 11296-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11296-2-AP (EIF2B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2B3 Polyclonal antibody (11296-2-AP)

    IF Staining of HeLa using 11296-2-AP

    Immunofluorescent analysis of HeLa cells using 11296-2-AP (EIF2B3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11296-2-AP targets EIF2B3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2B3 fusion protein Ag1809
Full Name eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa
Calculated Molecular Weight 44 kDa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC018728
Gene Symbol EIF2B3
Gene ID (NCBI) 8891
RRIDAB_2096142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF2B3 antibody 11296-2-APDownload protocol
IHC protocol for EIF2B3 antibody 11296-2-APDownload protocol
IF protocol for EIF2B3 antibody 11296-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Elife

The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.

Authors - Yao Liang Wong
View Article
humanIF

Mol Biol Cell

Cellular eIF2B subunit localization: implications for the integrated stress response and its control by small molecule drugs.

Authors - Rachel E Hodgson
View Article