Alpha Actin Polyclonal antibody
Alpha Actin Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11032-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, mouse thymus tissue |
| Positive IP detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
11032-1-AP targets Alpha Actin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Alpha Actin fusion protein Ag1471 |
| Full Name | actin, alpha, cardiac muscle 1 |
| Calculated Molecular Weight | 377 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC009978 |
| Gene Symbol | ACTC1 |
| Gene ID (NCBI) | 70 |
| RRID | AB_2242376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha Actin antibody 11032-1-AP | Download protocol |
| IP protocol for Alpha Actin antibody 11032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci MnSOD Lysine 68 acetylation leads to cisplatin and doxorubicin resistance due to aberrant mitochondrial metabolism. | ||
Stem Cells Dev A model of early human embryonic stem cell differentiation reveals inter- and intracellular changes on transition to squamous epithelium. | ||
Onco Targets Ther Clinical Significance and Oncogenic Activity of GRWD1 Overexpression in the Development of Colon Carcinoma. |
