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human IgM Polyclonal antibody

human IgM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11016-1-AP

Synonyms

human IgM, IGHM, IgM, MU, VH

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human plasma using human IgM Polyclonal antibody (11016-1-AP)

    WB analysis of human plasma using 11016-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 11016-1-AP (IGHM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Polyclonal antibody (11016-1-AP)

    IHC staining of human tonsillitis using 11016-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Polyclonal antibody (11016-1-AP)

    IHC staining of human tonsillitis using 11016-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11016-1-AP targets human IgM in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen human IgM fusion protein Ag1459
Full Name immunoglobulin heavy constant mu
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC009851
Gene Symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_593462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the heavy chain of human IgM (~75 kDa).

Protocols

Product Specific Protocols
WB protocol for human IgM antibody 11016-1-APDownload protocol
IHC protocol for human IgM antibody 11016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteomics

Tear dynamics testing and quantitative proteomics analysis in patients with chronic renal failure.

Authors - Rong-Rong Zong
View Article