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NOL3 Polyclonal antibody

NOL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10846-2-AP

Synonyms

Nop30, NOP, Myp, Muscle-enriched cytoplasmic protein, CARD2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NOL3 Polyclonal antibody (10846-2-AP)

    WB analysis of mouse skeletal muscle using 10846-2-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NOL3 Polyclonal antibody (10846-2-AP)

    WB analysis of mouse skeletal muscle using 10846-2-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NOL3 Polyclonal antibody (10846-2-AP)

    WB analysis of HeLa using 10846-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NOL3 Polyclonal antibody (10846-2-AP)

    IHC staining of human breast cancer using 10846-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10846-2-AP (NOL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NOL3 Polyclonal antibody (10846-2-AP)

    IHC staining of human breast cancer using 10846-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10846-2-AP (NOL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NOL3 Polyclonal antibody (10846-2-AP)

    IF Staining of HepG2 using 10846-2-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10846-2-AP (NOL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NOL3 Polyclonal antibody (10846-2-AP)

    IF Staining of HepG2 using 10846-2-AP

    Immunofluorescent analysis of HepG2 cells, using NOL3 antibody 10846-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10846-2-AP targets NOL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOL3 fusion protein Ag1306
Full Name nucleolar protein 3 (apoptosis repressor with CARD domain)
Calculated Molecular Weight 24 aa, 3 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC012798
Gene Symbol NOL3
Gene ID (NCBI) 8996
RRIDAB_2151850
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NOL3 antibody 10846-2-APDownload protocol
IHC protocol for NOL3 antibody 10846-2-APDownload protocol
IF protocol for NOL3 antibody 10846-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Biochim Biophys Acta Mol Basis Dis

Gut-derived 4-hydroxyphenylacetic acid attenuates sepsis-induced acute kidney injury by upregulating ARC to inhibit necroptosis

Authors - Sheng An
View Article
ratWB

Cell Death Dis

MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy.

Authors - X Li
View Article
mouseIF

Hum Genet

MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract.

Authors - Sandeep Aryal
View Article
mouseWB

Pharmacol Biochem Behav

Involvement of microglial cells in the antinociceptive effects of metamizol in a mouse model of neuropathic pain.

Authors - R Zajaczkowska
View Article
humanIHC

Front Genet

Molecular Characterization and Clinical Relevance of RNA Binding Proteins in Colorectal Cancer.

Authors - Zhen Zhang
View Article
humanWB

Cell Cycle

Long noncoding RNA PCAT6 inhibits colon cancer cell apoptosis by regulating anti-apoptotic protein ARC expression via EZH2.

Authors - Weimei Huang
View Article