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  • Featured Product
  • KD/KO Validated

BMI1 Polyclonal antibody

BMI1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10832-1-AP

Synonyms

RNF51, Polycomb group RING finger protein 4, Polycomb complex protein BMI-1, PCGF4

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using BMI1 Polyclonal antibody (10832-1-AP)

    WB analysis of HEK-293 using 10832-1-AP

    WB results of BMI antibody (10832-1-AP, 1:20000) with si-Control and si-BMI transfected HEK293 cells..

  • Western Blot (WB) analysis of U-937 cells using BMI1 Polyclonal antibody (10832-1-AP)

    WB analysis of U-937 using 10832-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using BMI1 Polyclonal antibody (10832-1-AP)

    WB analysis of K-562 using 10832-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using BMI1 Polyclonal antibody (10832-1-AP)

    WB analysis of NCCIT using 10832-1-AP

    NCCIT cell were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using BMI1 Polyclonal antibody (10832-1-AP)

    WB analysis of NCCIT using 10832-1-AP

    NCCIT cell were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using BMI1 Polyclonal antibody (10832-1-AP)

    WB analysis of mouse brain using 10832-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HT-29 cells using BMI1 Polyclonal antibody (10832-1-AP)

    IP experiment of HT-29 using 10832-1-AP

    IP result of anti-BMI1 (IP:10832-1-AP, 4ug; Detection:10832-1-AP 1:3000) with HT-29 cells lysate 1760 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BMI1 Polyclonal antibody (10832-1-AP)

    IHC staining of human liver cancer using 10832-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10832-1-AP (BMI1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BMI1 Polyclonal antibody (10832-1-AP)

    IHC staining of human liver cancer using 10832-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10832-1-AP (BMI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BMI1 Polyclonal antibody (10832-1-AP)

    IHC staining of human liver cancer using 10832-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10832-1-AP (BMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BMI1 Polyclonal antibody (10832-1-AP)

    IHC staining of human liver cancer using 10832-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10832-1-AP (BMI1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BMI1 Polyclonal antibody (10832-1-AP)

    IF Staining of HepG2 using 10832-1-AP

    Immunofluorescent analysis of HepG2 cells, using 10832-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BMI1 Polyclonal antibody (10832-1-AP)

    IF Staining of MCF-7 using 10832-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BMI1 antibody (10832-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BMI1 Polyclonal antibody (10832-1-AP)

    IF Staining of HepG2 using 10832-1-AP

    Immunofluorescent analysis of HepG2 cells, using IL34 antibody 10832-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inU-937 cells, HEK-293 cells, NCCIT cell, mouse brain tissue, K-562 cells
Positive IP detected inHT-29 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10832-1-AP targets BMI1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMI1 fusion protein Ag1286
Full Name BMI1 polycomb ring finger oncogene
Calculated Molecular Weight 37 kDa
Observed Molecular Weight37-43 kDa
GenBank Accession NumberBC011652
Gene Symbol BMI1
Gene ID (NCBI) 648
RRIDAB_2065392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.

Protocols

Product Specific Protocols
WB protocol for BMI1 antibody 10832-1-APDownload protocol
IHC protocol for BMI1 antibody 10832-1-APDownload protocol
IF protocol for BMI1 antibody 10832-1-APDownload protocol
IP protocol for BMI1 antibody 10832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Authors - Xu Wang
View Article
humanWB

Dev Cell

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Authors - Jie Ran
  • KD Validated
View Article
humanWB

Oncogene

Autophagy regulates the cancer stem cell phenotype of head and neck squamous cell carcinoma through the noncanonical FOXO3/SOX2 axis.

Authors - Yang Chen
View Article
mouseWB,CoIP,ChIP

J Neurosci

Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.

Authors - Kim Mee-Ohk MO
View Article
humanWB

Int J Cancer

SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.

Authors - S-L Xie
View Article
humanWB

Oncotarget

Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.

Authors - Xiaolong Wei
View Article