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COG1 Polyclonal antibody

COG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10767-1-AP

Synonyms

CDG2G, COG complex subunit 1, COG1, KIAA1381, LDLB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COG1 Polyclonal antibody (10767-1-AP)

    WB analysis using 10767-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10767-1-AP (COG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using COG1 Polyclonal antibody (10767-1-AP)

    WB analysis using 10767-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10767-1-AP (COG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using COG1 Polyclonal antibody (10767-1-AP)

    IHC staining of human ovary tumor using 10767-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10767-1-AP (COG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using COG1 Polyclonal antibody (10767-1-AP)

    IHC staining of human ovary tumor using 10767-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10767-1-AP (COG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inU-251 cells, PC-3 cells, U2OS cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10767-1-AP targets COG1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COG1 fusion protein Ag1203
Full Name component of oligomeric golgi complex 1
Calculated Molecular Weight 109 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC021985
Gene Symbol COG1
Gene ID (NCBI) 9382
RRIDAB_2083677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells (PMID: 16537452). It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa.

Protocols

Product Specific Protocols
WB protocol for COG1 antibody 10767-1-APDownload protocol
IHC protocol for COG1 antibody 10767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols