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  • Featured Product
  • KD/KO Validated

MBIP Polyclonal antibody

MBIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10685-1-AP

Synonyms

MUK-binding inhibitory protein, MUK binding inhibitory protein, MAPK upstream kinase-binding inhibitory protein, MAP3K12-binding inhibitory protein 1, BM-015

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MBIP Polyclonal antibody (10685-1-AP)

    WB analysis of HepG2 using 10685-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MBIP Polyclonal antibody (10685-1-AP)

    WB analysis of HEK-293 using 10685-1-AP

    WB result of MBIP antibody (10685-1-AP; 1:600; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using MBIP Polyclonal antibody (10685-1-AP)

    WB analysis of HEK-293 using 10685-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MBIP Polyclonal antibody (10685-1-AP)

    IP experiment of HEK-293 using 10685-1-AP

    IP result of anti-MBIP (IP:10685-1-AP, 4ug; Detection:10685-1-AP 1:300) with HEK-293 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using MBIP Polyclonal antibody (10685-1-AP)

    IHC staining of human testis using 10685-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MBIP Polyclonal antibody (10685-1-AP)

    IHC staining of human testis using 10685-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MBIP Polyclonal antibody (10685-1-AP)

    IHC staining of human lung cancer using 10685-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MBIP Polyclonal antibody (10685-1-AP)

    IHC staining of human lung cancer using 10685-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MBIP Polyclonal antibody (10685-1-AP)

    IF Staining of HepG2 using 10685-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10685-1-AP (MBIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10685-1-AP targets MBIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBIP fusion protein Ag1061
Full Name MAP3K12 binding inhibitory protein 1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC005197
Gene Symbol MBIP
Gene ID (NCBI) 51562
RRIDAB_2139889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

Protocols

Product Specific Protocols
WB protocol for MBIP antibody 10685-1-APDownload protocol
IHC protocol for MBIP antibody 10685-1-APDownload protocol
IF protocol for MBIP antibody 10685-1-APDownload protocol
IP protocol for MBIP antibody 10685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Characterizing the cancer genome in lung adenocarcinoma.

Authors - Weir Barbara A BA
  • KD Validated
View Article
HUMANWB

EMBO J

Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation.

Authors - Qihuang Jin
View Article
humanWB

Mol Cell Biol

The double-histone-acetyltransferase complex ATAC is essential for mammalian development.

Authors - Guelman Sebastián S
View Article
humanWB

Cell Signal

MBIP promotes ESCC metastasis by activating MAPK pathway

Authors - Yanchun Ma
View Article
humanWB

J Biol Chem

Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes.

Authors - Sela Dotan D
View Article
humanWB

J Biol Chem

Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein.

Authors - Yuan-Liang Wang
View Article