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AGPAT1 Polyclonal antibody

AGPAT1 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10601-1-AP

Synonyms

1 AGP acyltransferase 1, 1 AGPAT 1, 1 AGPAT1, AGPAT1, G15, LPAAT alpha, LPAATA, Protein G15

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of Raji cells using AGPAT1 Polyclonal antibody (10601-1-AP)

    IP experiment of Raji using 10601-1-AP

    IP result of anti-AGPAT1 (IP:10601-1-AP, 4ug; Detection:10601-1-AP 1:300) with Raji cells lysate 2000ug.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using AGPAT1 Polyclonal antibody (10601-1-AP)

    IP experiment of mouse skeletal muscle using 10601-1-AP

    IP result of anti-AGPAT1 (IP:10601-1-AP, 4ug; Detection:10601-1-AP 1:300) with mouse skeletal muscle tissue lysate 2200ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using AGPAT1 Polyclonal antibody (10601-1-AP)

    IHC staining of human skeletal muscle using 10601-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10601-1-AP (AGPAT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using AGPAT1 Polyclonal antibody (10601-1-AP)

    IHC staining of human skeletal muscle using 10601-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10601-1-AP (AGPAT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AGPAT1 Polyclonal antibody (10601-1-AP)

    IHC staining of human testis using 10601-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10601-1-AP (AGPAT1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AGPAT1 Polyclonal antibody (10601-1-AP)

    IHC staining of human testis using 10601-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10601-1-AP (AGPAT1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IP detected inRaji cells, mouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10601-1-AP targets AGPAT1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGPAT1 fusion protein Ag0837
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha)
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC003007
Gene Symbol AGPAT1
Gene ID (NCBI) 10554
RRIDAB_2224932
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for AGPAT1 antibody 10601-1-APDownload protocol
IP protocol for AGPAT1 antibody 10601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Metab

A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells

Authors - Shohei Matsufuji
View Article