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APOL4 Polyclonal antibody

APOL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10564-1-AP

Synonyms

ApoL-IV, Apolipoprotein L-IV, Apolipoprotein L4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOL4 Polyclonal antibody (10564-1-AP)

    WB analysis using 10564-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10564-1-AP (APOL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using APOL4 Polyclonal antibody (10564-1-AP)

    IP experiment of HepG2 using 10564-1-AP

    IP result of anti-APOL4 (IP:10564-1-AP, 4ug; Detection:10564-1-AP 1:2000) with HepG2 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using APOL4 Polyclonal antibody (10564-1-AP)

    IHC staining of human liver cancer using 10564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using APOL4 Polyclonal antibody (10564-1-AP)

    IHC staining of human liver cancer using 10564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using APOL4 Polyclonal antibody (10564-1-AP)

    IHC staining of human liver using 10564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using APOL4 Polyclonal antibody (10564-1-AP)

    IHC staining of human liver using 10564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOL4 Polyclonal antibody (10564-1-AP)

    IF Staining of HepG2 using 10564-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOL4 antibody (10564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOL4 Polyclonal antibody (10564-1-AP)

    IF Staining of HepG2 using 10564-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10564-1-AP (APOL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, U-87 MG cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10564-1-AP targets APOL4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOL4 fusion protein Ag0849
Full Name apolipoprotein L, 4
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC006276
Gene Symbol APOL4
Gene ID (NCBI) 80832
RRIDAB_2242901
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APOL4 antibody 10564-1-APDownload protocol
IHC protocol for APOL4 antibody 10564-1-APDownload protocol
IF protocol for APOL4 antibody 10564-1-APDownload protocol
IP protocol for APOL4 antibody 10564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols