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  • Immunohistochemistry (IHC) staining of mouse testis tissue using HOXD3 Polyclonal antibody (10496-1-AP)

    IHC staining of mouse testis using 10496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10496-1-AP (HOXD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HOXD3 Polyclonal antibody (10496-1-AP)

    IF Staining of U2OS using 10496-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HOXD3 antibody (10496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

HOXD3 Polyclonal antibody

HOXD3 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 10496-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

Hox 4.1, Homeobox protein Hox-D3, Homeobox protein Hox-4A, Homeobox protein Hox D3, Homeobox protein Hox 4A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10496-1-AP targets HOXD3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOXD3 fusion protein Ag0768 Predict reactive species
Full Name homeobox D3
Calculated Molecular Weight 46 kDa
GenBank Accession NumberBC005124
Gene Symbol HOXD3
Gene ID (NCBI) 3232
RRIDAB_2233224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31249
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homeobox D3 (HOXD3), a member of the Homeobox (Hox) family of master transcription factors, is a homeobox transcription factor that promotes angiogenesis and collagen synthesis. HOXD3 is expressed during normal embryogenesis, skin development, and fetal wound healing (PMID: 14633614). The overexpression of HOXD3 plays a critical role in breast cancer stemness and drug resistance via integrin β3-mediated Wnt/β-catenin signaling (PMID: 29698974).

Protocols

Product Specific Protocols
IHC protocol for HOXD3 antibody 10496-1-APDownload protocol
IF protocol for HOXD3 antibody 10496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols