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KD/KO Validated

p19 INK4d Polyclonal antibody

Name: p19 INK4d Polyclonal antibody
Brand: Proteintech
SKU: 10272-2-AP

p19 INK4d Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10272-2-AP

Synonyms

CDKN2D, INK4D, P19, p19 INK4D

Validation Data Gallery

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AllWBIHC

WB analysis using 10272-2-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10272-2-AP (p19 INK4d antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 10272-2-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10272-2-AP (p19 INK4d antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10272-2-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10272-2-AP (p19 INK4d antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10272-2-AP

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Tested Applications

Positive WB detected in	mouse testis tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 3 publications below

Product Information

10272-2-AP targets p19 INK4d in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	p19 INK4d fusion protein Ag0415
Full Name	cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
Calculated Molecular Weight	19 kDa
Observed Molecular Weight	18-20 kDa
GenBank Accession Number	BC001822
Gene Symbol	p19 INK4d/CDKN2D
Gene ID (NCBI)	1032
RRID	AB_2078742
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for p19 INK4d antibody 10272-2-AP	Download protocol
IHC protocol for p19 INK4d antibody 10272-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Blood Interconnecting molecular pathways in the pathogenesis and drug sensitivity of T-cell acute lymphoblastic leukemia. Authors - Sanda Takaomi T View Article
mouse	WB,IHC	Oxid Med Cell Longev Resveratrol Inhibits Ischemia-Induced Myocardial Senescence Signals and NLRP3 Inflammasome Activation. Authors - Hong Feng View Article
human	WB	FASEB J Overexpression of ERBB4 rejuvenates aged mesenchymal stem cell and enhances angiogenesis via PI3K/AKT and MAPK/ERK pathway. Authors - Xiaoting Liang View Article
human	WB	Front Immunol Possible Roles of Proinflammatory Signaling in Keratinocytes Through Aryl Hydrocarbon Receptor Ligands for the Development of Squamous Cell Carcinoma. Authors - Yota Sato View Article
human	WB	J Virol Rb-E2F-HDAC Repressor Complexes Control Interferon-Induced Repression of Adenovirus To Promote Persistent Infection. Authors - Sydney Snaider View Article
mouse	WB	Lab Invest Th17/IL-17 induces endothelial cell senescence via activation of NF-κB/p53/Rb signaling pathway. Authors - Liang Zhang View Article

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p19 INK4d Polyclonal antibody

p19 INK4d Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 10272-2-AP

IHC staining of mouse testis using 10272-2-AP

IHC staining of mouse testis using 10272-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Interconnecting molecular pathways in the pathogenesis and drug sensitivity of T-cell acute lymphoblastic leukemia.

Resveratrol Inhibits Ischemia-Induced Myocardial Senescence Signals and NLRP3 Inflammasome Activation.

Overexpression of ERBB4 rejuvenates aged mesenchymal stem cell and enhances angiogenesis via PI3K/AKT and MAPK/ERK pathway.

Possible Roles of Proinflammatory Signaling in Keratinocytes Through Aryl Hydrocarbon Receptor Ligands for the Development of Squamous Cell Carcinoma.

Rb-E2F-HDAC Repressor Complexes Control Interferon-Induced Repression of Adenovirus To Promote Persistent Infection.

Th17/IL-17 induces endothelial cell senescence via activation of NF-κB/p53/Rb signaling pathway.