Anticorps Monoclonal anti-Chromogranin A

Chromogranin A Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2A7D11

N° de cat : 60135-2-Ig

Synonymes

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

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Galerie de données de validation

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  WB analysis of mouse pancreas using 60135-2-Ig

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig pancreas using 60135-2-Ig

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig adrenal gland using 60135-2-Ig

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 60135-2-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 60135-2-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 60135-2-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 60135-2-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 60135-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60135-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60135-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60135-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas cancer using 60135-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human pancreas cancer using 60135-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of Neuro-2a using 60135-2-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed Neuro-2a cells using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, cellules BxPC-3, cellules Neuro-2a, cellules PC-12, cellules SH-SY5Y, tissu de glande surrénale de porc, tissu pancréatique de porc
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du pancréas humain, cellules Neuro-2a

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

60135-2-Ig cible Chromogranin A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Chromogranin A Protéine recombinante Ag0807
• Nom complet: chromogranin A (parathyroid secretory protein 1)
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC006459
• Symbole du gène: CHGA
• Identification du gène (NCBI): 1113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Publications

Species	Application	Title
mouse	IF	Stem Cell Res Ther; Expansion of mouse castration-resistant intermediate prostate stem cells in vitro; Authors - Yalan Xu
human	IF	Front Pharmacol; Development of a small cell lung cancer organoid model to study cellular interactions and survival after chemotherapy; Authors - Chandani Sen
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang