Anticorps Polyclonal de lapin anti-ZP3

ZP3 Polyclonal Antibody for IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (2)

N° de cat : 21279-1-AP

Synonymes

Sperm receptor, Zona pellucida glycoprotein 3, Zona pellucida protein C, Zp 3, ZP3, ZP3A, ZP3A/ZP3B, ZP3B, ZPC

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Galerie de données de validation

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  IP experiment of HeLa using 21279-1-AP

  IP result of anti-ZP3 (IP:21279-1-AP, 4ug; Detection:21279-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of mouse ovary using 21279-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21279-1-AP (ZP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 21279-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21279-1-AP (ZP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 21279-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21279-1-AP (ZP3 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of mouse ovary using 21279-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21279-1-AP (ZP3 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of mouse ovary using 21279-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using 21279-1-AP (ZP3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse ovary using 21279-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using 21279-1-AP (ZP3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu ovarien de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu ovarien de souris,

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

21279-1-AP cible ZP3 dans les applications de WB, IHC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZP3 Protéine recombinante Ag15791
• Nom complet: zona pellucida glycoprotein 3 (sperm receptor)
• Masse moléculaire calculée: 373 aa, 41 kDa
• Poids moléculaire observé: 47 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC113949
• Symbole du gène: ZP3
• Identification du gène (NCBI): 7784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that is processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).

Publications

Species	Application	Title
mouse	WB,CoIP	Cell Death Differ; Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.; Authors - Zhaokang Cui
human	IHC,IF,CoIP	Am J Hum Genet; A Recurrent Missense Mutation in ZP3 Causes Empty Follicle Syndrome and Female Infertility.; Authors - Tailai Chen
hamster	IF	Fertil Steril; The critical role of ZP genes in female infertility characterized by empty follicle syndrome and oocyte degeneration.; Authors - Ping Yang
mouse	WB,IF	Hum Reprod; Identification of a heterozygous variant of ZP2 as a novel cause of empty follicle syndrome in humans and mice.; Authors - Ying Shen
human	IF	Cell Discov; Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance.; Authors - Chunfang Liu
mouse	IF	Hum Mutat; A novel gene mutation in ZP3 loop region identified in patients with empty follicle syndrome; Authors - Zhiqiang Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Julia (Verified Customer) (08-18-2022)

It works well.

• Applications: Western Blot, Immunofluorescence

FH

Kathryn (Verified Customer) (04-22-2022)

This antibody works beautifully to highlight the zona pellucida on mouse ovary sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse ovary sections

ZP3 Antibody Immunofluorescence validation (1:100 dilution) in Mouse ovary sections (Cat no:21279-1-AP)

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