Anticorps Polyclonal de lapin anti-Thioredoxin

• Phare
• Validé par KD/KO

Thioredoxin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, FC (Intra), IP, CoIP, ELISA, IF

Conjugaison

Non conjugué

Publications

74

Avis (1)

N° de cat : 14999-1-AP

Synonymes

TXN, TRDX, THIO, Surface-associated sulphydryl protein, Hom s Trx

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Galerie de données de validation

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  WB analysis using 14999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14999-1-AP (Thioredoxin antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14999-1-AP (Thioredoxin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14999-1-AP

  WB result of Thioredoxin antibody (14999-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thioredoxin transfected HeLa cells.

• 

  IP experiment of HeLa using 14999-1-AP

  IP result of anti-Thioredoxin (IP:14999-1-AP, 4ug; Detection:14999-1-AP 1:6000) with HeLa cells lysate 1520 ug.

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  IHC staining of human liver cancer using 14999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14999-1-AP (Thioredoxin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14999-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14999-1-AP (Thioredoxin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 14999-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Thioredoxin (14999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K562, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:9000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 66 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• IP: See 5 publications below
• ELISA: See 1 publications below
• CoIP: See 3 publications below

Informations sur le produit

14999-1-AP cible Thioredoxin dans les applications de WB, IHC, FC (Intra), IP, CoIP, ELISA, IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Thioredoxin Protéine recombinante Ag6989
• Nom complet: thioredoxin
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC003377
• Symbole du gène: TXN
• Identification du gène (NCBI): 7295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Publications

Species	Application	Title
mouse	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
mouse	WB	Acta Pharm Sin B; Fluorine-thiol displacement probes for acetaminophen's hepatotoxicity; Authors - Benjamin L Prather
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
		Nano Converg; Detection of thioredoxin-1 using ultra-sensitive ELISA with enzyme-encapsulated human serum albumin nanoparticle.; Authors - Myeong-Jun Lee
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H
mouse	WB	Theranostics; TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.; Authors - Kailiang Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Amandine (Verified Customer) (02-07-2024)

20ug of protein, denatured or not, PVDF membrane, fast transfert, primary antibody 1/5000 1h30 RT, secondary antibody 1/5000 2h RT. Chemiluminescence revelation 10sec

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: ImCC

Thioredoxin Antibody Western Blot validation (1/5000 dilution) in ImCC (Cat no:14999-1-AP)

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