Anticorps Polyclonal de lapin anti-TPM2

• Phare
• Validé par KD/KO

TPM2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 11038-1-AP

Synonymes

AMCD1, Beta tropomyosin, DA1, DA2B, TMSB, TPM2, Tropomyosin 2, tropomyosin 2 (beta), Tropomyosin beta chain

---

Galerie de données de validation

• 

  WB analysis using 11038-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11038-1-AP (TPM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11038-1-AP

  WB result of TPM2 antibody (11038-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM2 transfected HeLa cells.

• 

  IP experiment of mouse skeletal muscle using 11038-1-AP

  IP result of anti-TPM2 (IP:11038-1-AP, 4ug; Detection:11038-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

• 

  IHC staining of human breast cancer using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11038-1-AP (TPM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11038-1-AP (TPM2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11038-1-AP (TPM2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11038-1-AP (TPM2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11038-1-AP (TPM2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11038-1-AP (TPM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11038-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPM2 antibody (11038-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidinv (red).

---

Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HeLa, muscle squelettique de rat, muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11038-1-AP cible TPM2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPM2 Protéine recombinante Ag1508
• Nom complet: tropomyosin 2 (beta)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 29-33 kDa
• Numéro d’acquisition GenBank: BC011776
• Symbole du gène: TPM2
• Identification du gène (NCBI): 7169
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPM2, also named as TMSB, belongs to the tropomyosin family. It binds to actin filaments in muscle and non-muscle cells. TPM2 plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. In non-piamuscle cells, TPM2 is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM2 are the cause of nemaline myopathy type 4 (NEM4) and distal arthrogryposis type 1 (DA1).

Publications

Species	Application	Title
human	WB	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
human,mouse	WB,IHC,IP	Cell Biosci; TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1; Authors - Zonglong Wu
mouse	WB	PLoS One; Proteomic changes in the hippocampus of large mammals after total-body low dose radiation; Authors - Diego Iacono
rat	WB	Int J Mol Sci; Modulation of Titin and Contraction-Regulating Proteins in a Rat Model of Heart Failure with Preserved Ejection Fraction: Limb vs. Diaphragmatic Muscle; Authors - Beatrice Vahle

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Marco (Verified Customer) (07-03-2024)

A view unspecific bands near the region of the protein, but enough blocking (o.N.) got rid of the problem. A well visible band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs